Written to be read withing the performance of Operature by ATOM-R.
Anatomy Lecture Theatre, Kings College London, 21st. October 2013.
Re-read at 6th Inter-format Symposium on Hybrid Natures 2016, Nida Art Colony.
Read #: liverwort/wind/code
Read 001
Liverwort scatters the subarctic tundra in vast dappling clusters of elephantine flaps of pale and palest green. A tundra is a biome where the tree growth is hindered by low temperatures and short growing seasons. Here the season is 100 days and the tundra begins at an entirely distinct level, the ecotone a clear mark where short crippled trees[1] as they are called give way entirely and absolutely, no gradual thinning, to where there are no trees, whatsoever.
But mosses. And Liverwort. Marchantia.
Read 002
He’s back up on the feet and he’s being very cautious. He gets a takedown, but is now dealing with a guillotine for a bit. He pops free. He was briefly in side control, but got up and went into the half guard. He is now in mount after a pass and looking to be real methodical. He is slowly crunching his opponent from side control.[2]
Read 003
We humans have more bacterial cells (1014) inhabiting our body than our own cells (1013) [2],[3]. It has been stated that the key to understanding the human condition lies in understanding the human genome [4],[5]. But given our intimate relationship with microbes [6], researching the human genome is now understood to be a necessary though insufficient condition: sequencing the genomes of our own microbes would be necessary too. Also, to better understand the role of microbes in the biosphere, it would be necessary to undertake a genomic study of them as well.[3]
Read 004
X-ray crystallography.
Diffraction of macromolecules
Here.
"the most beautiful X-ray photographs of any substance ever taken."[4]
He said.
Closer she came.
Read 005
Late one night
X-ray diffraction.
Failed
Again
Succession of failures
Read 006
It was a chilling experience, being in the hinterlands of a subarctic seemingly sparsely populated, only to discover numerous traces of war, WWII in particular, as we strode and hiked across the immediate surrounds. Gentle mossy dips revealing themselves to be dug out bunkers with rusted remnants still embedded and scattered; evidence of POW encampments, a WW2 Junnker crash site and an exploded WWI munitions storage facility - barely rusted, heaps of wire cutters, vast diaspora of casings and even some live ammunition, as well as heaps of safety pins.
Read 007
The liverworts (or marchantiophyta) are descendants of the earliest terrestrial plants. The group is characterised by morphological simplicity, and this seems to be matched by simple underlying genome structures. Liverworts show promise as new experimental systems after recent developments in transformation methods and genome characterisation.[5]
Read 008
He is back to standing. He has the back and is going for the Rear Naked Coke. He does get the Rear Naked Choke, while the other two carry own. He is in half guard with no kimura threat now.
He is standing as his opponent plays guard. He is being very calm and periodically going into top half and then returning to his feet. He has head control and gets swept by a keylock as he goes for a takedown. He is fending off halfhearted leglock attempts.[6]
Read 009
By providing the ability to examine the relationship of genome structure and function across many different species, these data have also opened up the fields of comparative genomics and of systems biology. Nevertheless, single organism genome studies have limits. First, technology limitations mean that an organism must first be clonally cultured to sequence its entire genome. However, only a small percentage of the microbes in nature can be cultured, which means that extant genomic data are highly biased and do not represent a true picture of the genomes of microbial species [10]–[12]. Second, very rarely do microbes live in single species communities: species interact both with each other and with their habitats, which may also include host organisms. Therefore, a clonal culture also fails to represent the true state of affairs in nature with respect to organism interaction, and the resulting population genomic variance and biological functions.[7]
Read 0010
Closer than most.
Here.
Between ‘51 and ‘53.
Photo 51.
In The Development of X-ray Analysis, Sir William Lawrence Bragg mentioned that he believed the field of crystallography was particularly welcoming to women because the techno-aesthetics of the molecular structures resembled textiles and household objects. Bragg was known to compare crystal formation to "curtains, wallpapers, mosaics, and roses."[8]
Read 0011
protein structures
folded
unfolded and
refolded
from
soluble
to unsoluble
unsolvable
improbabilities
of crystalline contingencies
Read 0012
We looked for Russian soldier burials, given directions by the headmaster of the local school, and dowsing for bones, found some, but we weren't certain of what species.
Read 0013
The relative simplicity of genetic networks in liverworts, combined with the growing set of genetic manipulation, culture and microscopy techniques, are set to make these lower plants major new systems for analysis and engineering.[9]
Read 0014
He is in mount after a fast pass to the left and is pressuring down in low mount. Looking to get a keylock going. He uses a burst of energy to try a pass, but he pulls him back to half guard, while yielding the flattened back. He gets bucked off and He is standing.[10]
Read 0015
New sequencing technologies and the drastic reduction in the cost of sequencing are helping us overcome these limits. We now have the ability to obtain genomic information directly from microbial communities in their natural habitats. Suddenly, instead of looking at a few species individually, we are able to study tens of thousands all together. Sequence data taken directly from the environment were dubbed the metagenome [13], and the study of sequence data directly from the environment—metagenomics [14].[11]
Read 0016
“Rosy, of course,
did not directly give us her data. For that matter, no one
at King’s realized they were in our hands.”[12]
Read 0017
Some proteins structures take no time at all, some take tens of years.
Some resist entirely and are unknowns and unknowables.
Read 0018
We rebuilt the crash event in our minds, visualising it's trajectory from a 13 year old witness account given to us by the now elderly man who houses that memory, and the expert opinion of the drone operators. Incorporations of metal and body, memory and remnant and, fresh evidence.
Read 0019
He moves to a back take/seatbelt control off the arm triangle position. They hit the ground after a guillotine attempt by him. He is besting from side control and he has a very deep Rear Naked Choke stuck in. He appears to be fighting it until the very last moment possible. Guillotine busted in and he is now working a mean guillotine from front headlock position. He lost the Rear Naked Choke.[13]
Rear 0020
Indexical double
Here
helices of coding
Here
Franklin’s persistence
here
of recording the reflections into Deoxyribonucleic acid resolutions.
Here
Kings here.
Here at Kings.
Read 0021
From these stories new shards were found, fresh, their placement in conciliation with our imaginings. We build model airplanes of Junnkers, enacted crashes, used toy drones and kites and found a quiet seriousness when burning the toy model on the crash site on a windy, rainy Sunday morning. Bodies exploded into nothing by their desperate payload.
Read 0022
He is on top of butterfly, but has the head controlled. They reset to middle. He works a mount to triangle. [14]
Read 0023
In contrast, the sequences obtained from environmental genomic studies are fragmented. Each fragment was obviously sequenced from a specific species, but there can be many different species in a single sample, for most of which a full genome is not available. In many cases it is impossible to determine the true species of origin. The length of each fragment can be anywhere between 20 base pairs (bp) and 700 bp, depending on the sequencing method used.[15]
Read 0024
The one that escaped, in a fever of burns and pure suffering.
‘Drones of Fascism’ one man remarked.
Read 0025
The liverworts have alternate haploid and diploid generations.[16]
Read 0026
He scrambles out.[17]
Read 0027
For these reasons, computational biologists have been developing new algorithms to analyze metagenomic data. These computational challenges are new and very exciting. We are entering an era akin to that of the first genomic revolution almost two decades ago. Whole organism genomics allows us to examine the evolution not only of single genes, but of whole transcriptional units, chromosomes, and cellular networks.[18]
Read 0028
They lie so low the continuous wind barely moves them. They lie with the wind. The reindeer turn into the wind,
Northerly.
Southerly.
And with them everything takes direction.
Read 0029
He is much shorter, but built like a barrel. He is trying to work from butterfly to an armbar or something without success. He is dancing around and he is being patient.[19]
Read 0030
Keeping strict and comprehensive records of metadata is as important as the sequence data. Metadata are the “data about the data”[20]
Read 0031
She wrote in Acta Crystallographica in September 1953 that
“discrepancies prevent us from accepting it in detail”[21]
Online sources quoted more than once:
ADCC 2013 Day 1 Live Stream Blog, By Ben Thapa. Retrieved on 21st October, 2013 from http://www.bloodyelbow.com/2013/10/18/4853132/adcc-2013-day-1-live-stream-blog-beijing-china-mendes-galvao-benson-braulio-bjj
A Primer on Metagenomics, John C. Wooley, Adam Godzik, Iddo Friedberg, PLOS, Computational Biology, retrieved on 21st October, 2013 from http://www.ploscompbiol.org/article/info%3Adoi%2F10.1371%2Fjournal.pcbi.1000667
Why Marcantia? Retrieved on 21st October 2013 from http://www.marchantia.org/home/why-marchantia.html
The Double Helix and the ‘wronged heroine’, Maddox, Brenda, Nature, vol 421, 2003. Retrieved from http://www.slideshare.net/ultramol/nature-review-rosalind-franklin-8381422
[1] Ella Tarvas’ comment that the word for crippled trees xxxxxx, carries more nuances than it’s English translation and referrers to small, elderly people, country people, poor and destitute kept together, crowded in what sounds like a workhouse.
[2] Adapted from ADCC 2013 Day 1 Live Stream Blog, By Ben Thapa.
[3] A Primer on Metagenomics, John C. Wooley, Adam Godzik, Iddo Friedberg, PLOS, Computational Biology.
[4] Attributed to pioneer of x-ray crystallography, John D. Bernal, retrieved on 21st October, 2013 from http://www.sdsc.edu/ScienceWomen/franklin.html
[5] Why Marchantia? Retrieved on 21st October 2013 from http://www.marchantia.org/home/why-marchantia.html
[6] Adapted from ADCC 2013 Day 1 Live Stream Blog, By Ben Thapa.
[7] A Primer on Metagenomics, John C. Wooley, Adam Godzik, Iddo Friedberg, PLOS, Computational Biology.
[8] Black, Susan AW (2005). "Domesticating the Crystal: Sir Lawrence Bragg and the Aesthetics of "X-ray Analysis"". Configurations 13 (2): 257. Retrieved on 1st October 2013 http://en.wikipedia.org/wiki/X-ray_crystallography#cite_ref-43
[9] Why Marchantia?
[10] Adapted from ADCC 2013 Day 1 Live Stream Blog, By Ben Thapa.
[11] A Primer on Metagenomics, John C. Wooley, Adam Godzik, Iddo Friedberg, PLOS, Computational Biology.
[12] James Watson being quoted by Brenda Maddox in The Double Helix and the ‘wronged heroine’, Maddox, Brenda, Nature, vol 421, pp 407-408.
[13] Adapted from ADCC 2013 Day 1 Live Stream Blog, By Ben Thapa.
[14] Ibid.
[15] A Primer on Metagenomics, John C. Wooley, Adam Godzik, Iddo Friedberg, PLOS, Computational Biology.
[16] Why Marchantia?
[17] Adapted from ADCC 2013 Day 1 Live Stream Blog, By Ben Thapa.
[18] A Primer on Metagenomics, John C. Wooley, Adam Godzik, Iddo Friedberg, PLOS, Computational Biology.
[19] Adapted from ADCC 2013 Day 1 Live Stream Blog, By Ben Thapa.
[20] A Primer on Metagenomics, John C. Wooley, Adam Godzik, Iddo Friedberg, PLOS, Computational Biology.
[21] The Double Helix and the ‘wronged heroine’, Maddox, Brenda, Nature, vol 421, 2003.
